human ovarian cancer cell lines caov3  (ATCC)

Journal: Cancer Drug Resistance

Article Title: ABCA1 is associated with the development of acquired chemotherapy resistance and predicts poor ovarian cancer outcome

doi: 10.20517/cdr.2020.107

Figure Lengend Snippet: ABCA1 mRNA expression is increased in ovarian cancer cells with acquired carboplatin resistance. (A) ABCA1 ; (B) ABCB1 ; (C) ABCB3 ; (D) ABCC2 ; and (E) ABCG2 expression in parental (OVCAR-5 and CaOV3) and carboplatin resistant ovarian cancer cell lines (OVCAR-5 CBPR and CaOV3 CBPR). For the cell line, the columns represent the mean fold changes ± SEM from 12-20 RNA samples from 4-6 independent experiments. * P < 0.05, unpaired t test. Data for the primary HGSOC cells from patients with chemosensitive at diagnosis ( n = 9) or acquired chemoresistance following relapse ( n = 6) are expressed as the median fold change from 3-6 RNA samples from two independent experiments. * P = 0.026, Mann-Whitney U test. ABCB1 was not detectable in OVCAR-5 cells.

Article Snippet: Human serous ovarian cancer cell line CaOV3 was purchased from American Type Culture Collection.

Techniques: Expressing, Biomarker Discovery, MANN-WHITNEY

Journal: Cancer Drug Resistance

Article Title: ABCA1 is associated with the development of acquired chemotherapy resistance and predicts poor ovarian cancer outcome

doi: 10.20517/cdr.2020.107

Figure Lengend Snippet: ABCA1 protein expression is upregulated in ovarian cancer cells with acquired carboplatin resistance. ABCA1 expression in OVCAR-5 (A) and CaOV3 (C) and carboplatin resistant OVCAR-5 CBPR (B) and CaOV3 CBPR (D) by immunocytochemistry using rabbit polyclonal ABCA1 antibody (1/100, NB400-105, Novus Biological). (E) OVCAR-5 cells with Rabbit IgG and (F) CaOV3 cells with Rabbit IgG. (G) Protein extracts from OVCAR-5 (~30 µg) and CaOV3 cell lines (~60 µg) were electrophoresed and immunoblotted with rabbit polyclonal ABCA1 antibody (1/1000, NB400-105, Novus Biological), and β-actin (1/2000, Abcam) was used as a loading control. A major band was detected at ~250 kDa, which is the predicted size for ABCA1. (H) Quantitation of ABCA1 Western blots. Data are from 2-4 independent experiments. Statistical significance was determined using the Student’s t -test, * P < 0.05.

Article Snippet: Human serous ovarian cancer cell line CaOV3 was purchased from American Type Culture Collection.

Techniques: Expressing, Immunocytochemistry, Control, Quantitation Assay, Western Blot

Journal: Cancer Drug Resistance

Article Title: ABCA1 is associated with the development of acquired chemotherapy resistance and predicts poor ovarian cancer outcome

doi: 10.20517/cdr.2020.107

Figure Lengend Snippet: Effects of apabetalone on ABCA1 expression and carboplatin response in ovarian cancer cells. (A) Apabetalone treatment reduced expression of ABCA1 in OVCAR-5 and OVCAR-5 CBPR cell lines. Cells were treated for 72 h with control medium (DMSO) or apabetalone (80 µM). Protein extracts from OVCAR-5 (~30 µg) were electrophoresed and immunoblotted with rabbit polyclonal ABCA1 antibody (1/1000, NB400-105, Novus Biological) and β-actin (1/2000, Abcam) was used as a loading control. (B) Quantification of Western blot. OVCAR-5 (C), CaOV3 (D), OVCAR-5 CBPR (E) and CaOV3 CBPR (F) cell survival following treatment with carboplatin (CBP) alone (0-200 µM, black line) and in combination with apabetalone (Apa, 80 µM, purple line). (G) Carboplatin IC 50 for OVCAR-5 and OVCAR-5 CBPR cells ± apabetalone. Data are mean ± SEM from three independent experiments. * P < 0.05, Student’s t -test. (H) Carboplatin IC 50 for CaOV3 and CaOV3 CBPR cells ± apabetalone. Data are mean ± SEM from three independent experiments. P < 0.05, Student’s t -test.

Article Snippet: Human serous ovarian cancer cell line CaOV3 was purchased from American Type Culture Collection.

Techniques: Expressing, Control, Western Blot